Purification and characterization of a heat-stable alkaline protease from Bacillus stearothermophilus F1
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چکیده
منابع مشابه
Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملThermostable alkaline protease from Bacillus stearothermophilus F1; nutritional factors affecting protease production
The production of thermostable extracellular protease by Bacillus stearothermophilus F1 was influenced by the composition of culture medium. Peptone iv derived from soybean was the best organic compound for enzyme production. Sodium nitrate, ammonium salts and amino acids as sole nitrogen sources interfered with protease formation. The addition of most carbon sources to the peptone medium faile...
متن کاملPurification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001.
The newly isolated alkalophilic Bacillus sp. B001 produced a high level of proteolytic activity (34277 U/mL) when grown in production medium, and a 28 kDa protease, designated AprB, was purified from the culture supernatant. Partial amino acid sequences were obtained by tandem mass spectrometry (MS/MS) and a pair of degenerate primers was developed to amplify a 467-bp genomic sequence. The obse...
متن کاملisolation, purification and characterization of a thermophilic alkaline protease from bacillus subtilis bp-36
the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...
متن کاملPurification and Characterization of Alkaline Protease from a Mutant Bacillus licheniformis Bl8
Alkaline protease from a mutant of Bacillus licheniformis Bl8 was purified from the culture supernatant by employing the methods such as ammonium sulphate precipitation, DEAE cellulose chromatography followed by Gel filtration using Sephadex G-100. The yield of the enzyme after purification was found to be 10%. Protease was found to be homogenous when examined by SDS-PAGE and the enzyme showed ...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 1994
ISSN: 0175-7598,1432-0614
DOI: 10.1007/bf00173982